Catalyst Awardee

Project Description

Construction and application of an efficient circulating tumor cell detection system based on magnetic cell probes

Fan Zhang, PhD | Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College; Lin Mei, PhD; Jinxie Zhang, PhD
Competition Sponsor:
 Chinese Academy of Medical Sciences
Awardee Year: 2023

Circulating tumor cells (CTCs) testing is important for the early diagnosis and treatment of cancer. However, in clinical application, the CTCs amount in blood is very low, the sample demand is large, the detection time is long, the epithelial-interstitial transition, the non-specific adsorption of white blood cells, and the cell loss is serious, which cannot meet the requirements of efficient sorting, enrichment, detection and analysis of CTCs.
In this project, Fe3O4 nanoclusters were phagocytized by monocytes which expressing epithelial cell adhesion molecule (EpCAM) and cell surface vimentin. And the magnetic cells targeting EpCAM and CSV antigens were prepared for capture circulating tumor cells. By combining magnetic cells with high-throughput microfluidic chips to construct an efficient circulating tumor cell detection system, CTCs detection and analysis with low sample size, simple operation, low adsorption of white blood cells and maintenance of cell activity can be realized efficiently. We would investigate the effect of the detection system on capturing simulated blood samples and CTCs in blood samples. By optimizing the preparation technology and detection scheme, it would obtain an efficient circulating tumor cell detection system based on magnetic cell probe, realize the capture of CTCs with low sample size, simple operation, high throughput, low adsorption white blood cells and high activity. This work would provide theoretical basis and experimental support for the capture, detection, culture and analysis technology of CTCs in scientific research and clinical practice.

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